发酵过程生化指标检测的自动取样设计

doi:10.3976/j.issn.1002-4026.2015.06.010

Abstract

Abstract: We design an online automatic sampling device and its control process for fermentation samples, which can collect samples in multiple fermentation tanks in sequence and time division to automatically determine the biochemical parameters of multiple fermentation processes. The device can support direct cell free sampling using filtering membrane or osmotic membrane sampling probes, direct sampling with cells using straight tube, and indirect sampling using Utube witch could simulate manual sampling. We present sampling processes and their software flowcharts of various sampling methods. For direct sampling with cells using straight tube, we also devise multiple bacteria antipollution barriers and provide method of sampling tube chemical sterilization process at the determination interval to prevent external bacteria pollution through sampling tube during longperiod fermentation. Combined with biosensor analysis system, the device can conduct online automatic analysis for biochemical parameters in different fermentation processes.

Key words: automatic sampling, antipollution sampling, biochemical parameters online detection

CLC Number:

Q815

ZHU Sirong, ZHOU Wanli, ZHANG Liqun, YANG Yan, GAO Guangheng, ZHAO Xiaohua. Automatic sampling design of fermentation biochemical indexes detection[J].SHANDONG SCIENCE, 2015, 28(6): 58-64.

References

［1］张先恩. 生物传感器［M］. 北京: 化学工业出版社, 2006: 325-334. ［2］PICQUE D, CORRIEU G. Performances of aseptic sampling devices for online monitoring of fermentation processes ［J］. Biotechnology and Bioengineering , 1992, 40(8):919-924 ［3］高学金,薛吉星,张鹏,等. 在线测量发酵液葡萄糖浓度的过滤取样探头的研制［J］. 分析化学, 2014,42(6):924-928. ［4］冯德荣,朱思荣,周万里, 等. SBA60型四电极生物传感分析系统的研制［J］. 山东科学, 1998, 11(2): 32-36 ［5］杨海麟,吕霞付,王龙刚, 等. 生物反应过程在线检测系统的研制［J］. 无锡轻工业大学学报,2003,22(1):33-37. ［6］杨海麟,吕霞付,王龙刚, 等. 发酵过程在线检测系统在谷氨酸发酵中的应用研究［J］. 中国调味品,2003(10):16-20. ［7］高学金,刘广生,程丽, 等. 发酵过程葡萄糖在线检测系统的研制［J］. 分析化学, 2012,40(12):1945-1949. ［8］LIU H, CHEN X G, WU L,et al. Development of the online automatic measuring system for total sugar content in chlortetracycline fermenter［J］. Advanced Materials Research , 2013, 760/761/762:1067-1070. ［9］BUTTLER T, GORTON L, JARSKOG H, et al. Monitoring of ethanol during fermentation of lignocellulose hydrolysate by online microdialysis sampling, column liquid chromatography.and an alcohol biosensor［J］. Biotechnology and Bioengineering, 1994, 44(3):322-328. ［10］HANSEN K F, LAURITSEN F R, DEGN H. An online Sampling ystem for fermentation monitoring using membrane inlet mass spectrometry(MIMS):Application to phenoxyacetic acid monitoring in penicillin fermentation［J］. Biotechnology and Bioengineering, 1994, 44(3):347-353. ［11］SHU H C, HAKANSON H, MATTIASSON B. Online monitoring of Dlactic acid during a fermentation process using immobilized Dlactate dehydrogenase in a sequential injection analysis system［J］. Analytica Chimica Acta, 1995, 300(1/2/3):277-285. ［12］van de MERBEL N C, KOOL I M, LINGEMAN H, et al. Online monitoring of fermentation processes by ultrafiltration and column liquid chromatography［J］. Chromatographia, 1992, 33(11): 525-532. ［13］LIDN H, BUTTLER T, JEPPSSON H, et al. Online monitoring of monosaccharides and ethanol during a fermentation by microdialysis sampling, liquid chromatography and two amperometric biosensors［J］. Chromatographia. 1998, 47(9/10):501-508. ［14］Flownamics Analytical Instruments, Inc. SegFlow4800 Automated Sampling & Feed Control System［EB/OL］.［2015-03-12］.http://www.systemcindustrie.com/bioprocess/wpcontent/uploads/segflowsamplemodule.pdf

Automatic sampling design of fermentation biochemical indexes detection

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Metrics

Comments

Recommended 0